Growth Factors Supplementation in Culture Medium Leads to Active Proliferation of Porcine Fibroblasts
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- 2016.04.01
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- 2011.09
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서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 35권 / 3호
ㆍ저자명 : Bella Kim, Nayoung Ko, Seong-soo Hwang, Gi-Sun Im, Dong-Hoon Kim, Jin-Ki Park, Zae Young Ryoo, Keon Bong Oh
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS Isolation of Porcine Fibroblasts Porcine Fibroblasts Culture for Proliferation Assay WST-1 Cell Proliferation Assay Coomassie Blue Staining of Fibroblast RESULTS AND DISCUSSION REFERENCES
영어 초록
Fibroblasts of large animals are easy to isolate and to maintain in vitro culture. Thus, these cells are extensively applied to donor cell for somatic cell nuclear transfer, and to substrate cells to generate induced pluripotent stem cells after transfection of required genes to be essentially required for direct reprogramming. However, limited mitotic activity of fibroblasts to differentiate along a terminal lineage becomes restrictive for their versatile application. Recently, commercial culture medium and systems developed for primary cells are provided by manufactures. In this study, we examined whether one of the systems developed for primary fibroblasts of human are effective on porcine ear skin fibroblasts. To this end, we performed proliferation assay after five days culture in vitro of porcine fibroblasts in medium DMEM, which is generally used for fibroblasts culture, and medium M106 for human dermal fibroblasts, supplemented with various concentrations of FBS and LSGS contained mainly growth factors, respectively. Consequence was that presence of 15% FBS and 0.1 X concentrations of LSGS in DMEM showed most active proliferation of porcine fibroblasts.
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