Generation of Reactive Oxygen Species in Porcine Parthenogenetic Embryos
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서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 35권 / 2호
ㆍ저자명 : In-Sun Hwang, Choon-Keun Park, Boo-Keun Yang, Hee-Tae Cheong
ㆍ저자명 : In-Sun Hwang, Choon-Keun Park, Boo-Keun Yang, Hee-Tae Cheong
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS In Vitro Maturation (IVM) of Oocytes Parthenogenesis and In Vitro Culture Analysis of ROS Products Experimental Designs Statistical Analysis RESULTS ROS Levels at Activation Procedures ROS Levels by the Time Elapsed after Electric Stimulation ROS Levels of Parthenogenetic Embryos during In Vitro Culture DISCUSSION REFERENCES영어 초록
The present study was conducted to examine the reactive oxygen species (ROS) generation levels in porcine parthenogenetic embryos. Porcine in vitro matured oocytes were activated by the combination of electric stimulus and 6‐ DMAP before in vitro culture. Porcine oocytes and parthenogenetic embryos were stained in 10 μM dichlorohydrofluorescein diacetate (DCF) or 10 μM hydroxyphenyl fluorescein (HPF) dye each for 30 min at 39℃. The fluorescent emissions from the samples were recoded as JPEG file and the intensity of fluorescence in oocytes and embryos were analyzed. H2O2 and ˙OH radical levels of porcine oocytes were reduced immediately after electric stimulation. However, H2O2 and ˙OH radical levels of parthenogenetic embryos were increased with time elapsed after electric stimulation from 0 h to 3 h and after DMAP culture. During in vitro culture, H2O2 and ˙OH radical levels were gradually increased from the one‐cell stage to the two‐ and four‐cell stages. The result of the present study suggests that the ROS was not increased by electric pulse in porcine embryos. Rather than it seems to be associated with the stage of development and the culture condition.참고 자료
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