Effects of Serum Addition and Different Culture Media on Growth of Porcine Preantral Follicles In Vitro
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서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 34권 / 3호
ㆍ저자명 : Yun-Fei Diao, Hong-Rye Kim, Rong-Xun Han, Myung-Yoon Kim, Chang-Sik Park, Dong-Il Jin
ㆍ저자명 : Yun-Fei Diao, Hong-Rye Kim, Rong-Xun Han, Myung-Yoon Kim, Chang-Sik Park, Dong-Il Jin
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS Regents Animals Preantral Follicle Dissection and Collection Preantral Follicle Culture In Vitro Experimental Design Statistical Analysis RESULTS Effects of FCS on Porcine Preantral Follicular Survival, Growth and Antumn Formation Effects of Different Culture Media (MI99 and NC-SU23) on Survival, Growth and Antrum Formation of Porcine Preantral Follicles DISCUSSION REFERENCES영어 초록
Current developments in IVF and animal cloning have resulted in increasing demand for large quantities of oocytes and ovarian follicles at specific stages of development. These medical and scientific needs may be met by developing an optimal culture system for preantral follicles. In this study, we investigated the growth of porcine preantral follicle cultures in different media and in the presence and absence of serum. Follicles were manually dissected from ovaries obtained from prepubertal gilts at a local slaughterhouse, and cultured for 3 days in M199 or NCSU23 medium supplemented with porcine FSH, transferrin, L-ascorbic acid and insulin. Follicle diameters were measured on day 1 and 3 of culture. In Experiment 1, the effect of supplementing culture medium with fetal calf serum (FCS) on porcine preantral follicle growth was examined. In the group of cultures supplemented with FCS, follicle diameter after 3 days of culture, survival rate and antrum formation rate in the FCS group were significantly higher than those of the control group. In Experiment 2, the effects of culture medium (M199 and NCSU23) on follicle growth were compared. Follicle diameters were increased in the M199 group, compared with those in NCSU23 (p<0.05), but we observed no significant differences in survival and antrum formation rates between cultures grown in the two media. In conclusion, supplementation of the culture medium with serum enhances preantral follicle growth and antrum formation, and M199 is superior to NUSU23 for porcine preantral follicle culture in vitro.참고 자료
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