Expression of Placenta-Related Genes (Cdx2 and GATA6) in Cloned Porcine Development
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서지정보
ㆍ발행기관 : 한국동물번식학회
ㆍ수록지정보 : Reproductive & developmental biology / 33권 / 4호
ㆍ저자명 : Byung-Hyun Cha, Seongsoo Hwang, Hwi-Cheul Lee, Mi-Rung Park, Gi-Sun Im, Jae-Seok Woo, Soo-Bong Park, Jae-Hyeon Cho, Yeoung-Gyu Ko
ㆍ저자명 : Byung-Hyun Cha, Seongsoo Hwang, Hwi-Cheul Lee, Mi-Rung Park, Gi-Sun Im, Jae-Seok Woo, Soo-Bong Park, Jae-Hyeon Cho, Yeoung-Gyu Ko
목차
ABSTRACT INTRODUCTION MATERIALS AND METHODS RESULTS DISCUSSION REFERENCES영어 초록
Abnormal development and fetal loss during the post‐implantation period are key concerns in the production of cloned animals by somatic cell nuclear transfer (SCNT). We hypothesized that the problems in cloned porcine offspring derived from SCNT are related to interactions between the conceptus and the endometrial environment. In the present study, we investigated expression patterns in the formation of placenta‐related genes (Cdx2 and GATA6) in whole in vivo normal porcine embryos (from single cell to blastocyst) and each tissue of a normal fetus at Days 25, 35 and 55 by quantitative mRNA expression analysis using real‐time PCR. The expression of Cdx2 and GATA6 mRNA increased to around the blastocyst stage. These genes were gradually decreased from the peri‐implantation to post‐implantation stage. Moreover, we examined the expression patterns of Cdx2 and GATA6 in Day 35 normal and SCNT cloned fetuses by the same methods. And, the level of Cdx2 and GATA6 gene expression in the extraembryonic tissue of SCNT was significantly higher than that of control tissues. From the present results, it can be postulated that the aberrant expression of Cdx2 and GATA6 genes in the endometrial and extraembryonic tissues at pre‐ and peri‐implantation stages may be closely related to the lower fficiency of animal cloning.참고 자료
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