Alteration of DNA Methylation in Oct-4 Gene in Mouse Preimplanation Embryos by the Interference RNA

서지정보

ㆍ발행기관 : 한국동물번식학회 ㆍ수록지정보 : Reproductive & developmental biology / 31권 / 1호
ㆍ저자명 : Jong-Mu Kim, Yeoung-Gyu Ko, Hwan-Hoo Seong, Hak-Jae Chung, Won-Kyong Chang, Nam-Hyung Kim

목차

ABSTRACT INTRODUCTION MATHRIALS AND METHODS Coiiection of Mouse Embryo and Sperm RNAi Injection Genomic DNA Preparation DNA Methylation Analtsis followed by Bi-sulfite Sequencing Real Time RT-PCR RESULTS DNA Methylation Statue of the 5'Flanking Region of the Oct-4 Gene Specific of Oct-4 mRNA by siRNA Injection Alterration of Methylation Patterns by siRNA Injection DISCUSSION REFERENCES

영어 초록

During early embryo development, Oct-4 is an important transcription factor for the early differentiation. The present study was first examined methylation status in distal enhancer and promoter region of Oct-4 during mouse pre-implantation embryo development. In oocyte and sperm, high methylation was observed in both distal and proximal of promoter in Oct-4. Following fertilization, relatively high methylation level remained until 8-cell stage embryos, but decreased at the morula and blastocyst stage. Specific gene knock down of Oct-4 by siRNA injection into zygote induced higher methylation rates of both distal and proximal region ofpromoter of Oct-4. These results suggest a functional link between the DNA methylation status of distal and promoter region in the Oct-4 gene and the gene sequence-specific transcriptional silencing by exogenous siRNA injection during mouse pre- implantation embryos.

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