천연물생리활성방법론

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2. 천연물로부터 항암물질을 찾고자 일차 스크리닝을 실시하고자 할 때 응용될 수 있는 in vitro 실험법을 열거하고 그중 한 예를 들어 간략히 실험법에 대하여 기술하라. (Enumerate in vitro test methods that can be applied to find the anticancer agents from natural products and briefly describe one experimental method)
Answer:
I
n vitro bioassay for screening anti-cancer agents from natural sources:

1. MTT assay
2.

MTT assay: The MTT assay is used to measure cellular metabolic activity as an indicator of cell viability, proliferation and cytotoxicity. This colorimetric assay is based on the reduction of a yellow tetrazolium salt (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide or MTT) to purple formazan crystals by metabolically active cells. The viable cells contain NAD(P)H-dependent oxidoreductase enzymes which reduce the MTT to formazan.36 The insoluble formazan crystals are dissolved using a solubilization solution and the resulting colored solution is quantified by measuring absorbance at 500-600 nanometers using a multi-well spectrophotometer.

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